Fig. 1.

Synuclein characterization by immunofluorescence. 1 (a)-, 2 (b)-, and 3 (c)-week-old cultures stained for α-synuclein (red) and synaptophysin (green). Cytosolic α-synuclein staining is evident in a.d, Double labeling of α-synuclein (green) and GAD (red) show colocalization in an inhibitory neuron and in GABAergic presynaptic terminals (yellow). e, α- and β-synuclein are colocalized (yellow) to the presynaptic terminal. f, γ-synuclein (red) is poorly expressed in mature hippocampal neurons as compared to synaptophysin (green). The nuclei in a–f are labeled by Hoechst (blue) dye. Scale bar, 10 μm.