Table 1.
PDH-immunoreactive neurons in young (2–10 d) and old (20–30 d) flies of the different gal4 lines
| Description of cell clusters | elav-gal4 | Mz1366-gal4 | Mz1525-gal4 | Mz1172-gal4 | per-gal4 | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Y | Old | Y | Old | Y | Old | Y | Old | Y | Old | |
| Anterior brain | ||||||||||
| LNv (large and small) | + | + | − | − | + | + | − | − | + | + |
| 1–3 LNd | + | + | − | − | (+) | − | − | − | + | (+) |
| DGI | ++ | + | − | − | (+) | − | + | + | ++ | + |
| Two neurons ventral to LNv(1) | + | + | + | (+) | − | − | − | − | + | (+) |
| One to four small neurons dorsal to LNv(2) | + | (+) | + | − | (+) | − | − | − | − | − |
| α-, β-, γ-lobe of the mushroom body | ++ | ++ | − | − | − | − | − | − | − | − |
| Glomeruli of the AL | ++ | ++ | − | − | ++ | ++ | − | − | − | − |
| Many small neurons dorsolateral to the AL (3) | + | + | − | − | + | + | − | − | − | − |
| Three to eight small neurons lateral of the EF, ventromedial to the AL (4) | + | + | + | + | ++ | + | − | − | (+) | − |
| One lateral cell in the tritocerebrum (5) | + | + | (+) | − | (+) | − | (+) | − | − | − |
| Up to 20 neurons in the anterior medial subesophageal ganglion (SE) (6) | + | + | ++ | + | + | − | + | (+) | + | − |
| Medial brain | ||||||||||
| Up to 10 neurons ventrally in the SE (7) | + | + | + | − | + | (+) | + | − | + | − |
| One large neuron in the lateral and ventral brain (8) | ++ | ++ | ++ | ++ | ++ | ++ | − | − | − | − |
| Up to 10 cells in the pars intercerebralis (9) | + | + | ++ | ++ | + | − | ++ | ++ | (+) | − |
| One layer in the fan shaped body | ++ | ++ | + | − | − | − | (+) | − | − | − |
| Posterior brain | ||||||||||
| Three to 10 DN1 | + | + | − | − | + | − | + | − | + | + |
| One to two DN2 | (+) | − | − | − | (+) | − | + | − | + | (+) |
| Large neuron in the posterior pars lateralis (PL) close to the terminals of the small LNv(10) | + | + | − | − | − | − | ++ | ++ | + | − |
| Second large neuron in the posterior PL (11) | + | + | ++ | ++ | + | + | − | − | − | − |
| One neuron at the level of the ellypsoid body that projects dorsally (12) | + | + | − | − | + | + | − | − | − | − |
| Huge neuron in the lateral posterior brain dorsal to the POT (13) | ++ | + | ++ | + | + | (+) | + | (+) | + | (+) |
| Three to eight small neurons close to the huge neuron (14) | + | + | ++ | + | + | + | + | (+) | (+) | − |
| Several small neurons scattered in the posterior brain (not numbered) | (+) | (+) | (+) | − | (+) | − | − | − | − | − |
| Thoracic nervous system | ||||||||||
| Up to four large neurons ventrally in the midline between segment 1 and 2 | + | + | + | − | + | − | + | (+) | − | − |
| One to four small neurons ventrally in the midline of segments 1 and 3 and between both | + | (+) | (+) | (+) | (+) | − | + | + | − | − |
| Large and small cells at the margin of the thoracic NS | + | (+) | + | − | (+) | − | (+) | − | − | − |
| Additional cells in the abdominal segments | + | + | ++ | + | + | − | ++ | ++ | − | − |
| Horseshoe-shaped cell group located superficially in the dorsal part of segment 3 | + | + | + | (+) | (+) | − | ++ | ++ | − | − |
Y, Young; −, no staining; (+), sometimes weak staining; +, always staining; ++, always strong staining.