Fig. 3.
Translation of zebrafish connexin cRNAs in microinjected Xenopus oocytes and reticulocyte lysate. Experimental conditions are indicated on top of each lane. Xenopus oocytes were injected with 40 nl of either water or cRNAs (∼40–60 ng per cell) coding for retinal zebrafish connexins and incubated in the presence of [35S]methionine (0.5 mCi/ml). Translated products and labeled proteins from oocyte homogenates were separated on a 13% SDS-polyacrylamide gel and detected by fluorography. The migration of protein standards is shown in lane 5 with their molecular mass (in kilodaltons) indicated on the right edge of the gel.