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Cd2+ (200 μm) blocks both the primary and secondary spike-associated [Ca2+]i increase in the presence of 30 μmt-ACPD. The middle panelshows that Cd2+ did not affect the resting fluorescence level in the cell, indicating that Cd2+did not enter the cell to quench the indicator fluorescence. Scale is in arbitrary units. The bottom panels show representative responses. A small spike-associated response, insufficient to evoke release, remained in Cd2+-containing ACSF.
