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. 2019 Jul 29;37(9):1176–1188. doi: 10.1002/stem.3039

Figure 6.

Figure 6

In utero transplantation (IUT) of cultured amniotic fluid stem cells (AFSCs) results in long‐term multilineage hematopoietic engraftment. (A): Experimental design for IUT of fresh and cultured AFSC (B6‐GFP; CD117+, Lin−, H2Kb+; 104 per fetus), performed at E14. Chimerism was checked up to 24 weeks post‐IUT. (B): Fetal survival to birth after IUT of fresh versus cultured AFSC. (C): Four‐week chimerism analysis showed 100% engraftment in both groups (representative FACS histograms; fresh AFSC: 14/14; cultured AFSC: 13/13) with similar levels of macrochimerism (representative FACS histograms). (D): Engraftment levels between fresh (n = 14) versus cultured AFSC (n = 13) at 4, 12, 24 weeks post‐IUT. (E, F): Multilineage reconstitution of transplanted AFSC (fresh and cultured) compared with donor lineages (representative FACS histograms; n = 8 per group). (G, H): Hematopoietic gene array analysis comparing donor‐derived (fresh or cultured) AFSC and host bone marrow‐derived hematopoietic stem cells (representative heat maps; three independent experiments). Black bars: fresh AFSC/fresh AFSC‐derived; gray bars: cultured AFSC/cultured AFSC‐derived; white bars: host‐derived.