Skip to main content
. 2019 Aug 27;18(20):2713–2726. doi: 10.1080/15384101.2019.1656476

Figure 1.

Figure 1.

Depletion of α-fodrin leads to abnormal mitosis. (a) Blot showing downregulation of α-fodrin in U-251 MG cell line using single shRNA targeted against fodrin (shRNA a) and mixture of 3 shRNA (shRNA b) with actin as loading control. (b) U-251 MG cells were subjected to α-fodrin downregulation using shRNA for 96 h and cells were fixed using ice cold methanol. They were then stained with α-tubulin antibody and nuclei were stained with DAPI. Quantitative analysis of the percentage of mitotic cells in control and fodrin shRNA-treated cells using shRNA a. (c) Mitotic abnormalities observed in case of fodrin depleted cells using shRNA b. The experiment was repeated thrice. (d) Quantitative analysis of the percentage of normal, misaligned and multipolar cells in control and fodrin shRNA-treated cells. N = 125 and the experiment was repeated thrice. (e) Western blot showing no change in tubulin and actin levels upon fodrin depletion by shRNA with a GAPDH as loading control. For statistical analysis, Student's t-test was carried out and * represents p < 0.05 and *** represents p < 0.001. Scale bar represents 5 μm.