Figure 5.
Depletion of centrobin has a defect in UV-induced microtubule stabilization. (a) Experimental scheme. HeLa cells were transfected with the indicated siRNAs. At 48 hr after transfection, cells were either left untreated or treated with 15 J/m2 UV and then incubated for 20 h. Before staining, siRNA-treated HeLa cells were treated with 2 μM nocodazole for 45 min. (b) Representative immunofluorescence (IF) images of siRNA-treated HeLa cells stained for α-tubulin (red), acetylated α-tubulin (green), and DNA (blue). Scale bar, 10 μM.