Figure 2.

Silencing of SBF2-AS1 restrains proliferation, migration and invasion and contributes to apoptosis of OS cells. (a,b) Detection of SBF2-AS1 expression in U-2OS cells by RT-qPCR; (c) Detection of cell viability by MTT assay; (d) Detection of cell colony formation rate by colony formation assay; (e) Detection of cell apoptosis by flow cytometry; (f) Detection of cell migration rate in each group by Transwell assay; (g) Detection of cell invasion in each group by Transwell assay; * P < 0.05 vs the sh-NC group; #, P < 0.05 vs the oe-NC group; the data were all measurement data and expressed as mean ± standard deviation; independent sample t test was used for statistical analysis between two groups, and one-way ANOVA for the comparison among multiple groups, followed by Tukey’s post hoc test; the experiment was repeated three times.