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. 2019 Aug 21;18(20):2727–2741. doi: 10.1080/15384101.2019.1656478

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Figure 3. — LncRNA SBF2-AS1 regulates miR-30a by acting as a sponge. (a) Prediction of SBF2-AS1 subcellular localization by online analysis website; (b) Verification of SBF2-AS1 subcellular localization by FISH assay; (c) Prediction of the binding site of SBF2-AS1 to miR-30a by RNA22 website; (d) Verification of the binding site of SBF2-AS1 and miR-30a by double luciferase reporter gene assay; (e) Detection of the binding of SBF2-AS1 and Ago2 by RIP assay; (f) Enrichment of miR-30a to SBF2-AS1 by RNA pull-down assay; *, P < 0.05 vs the oe-NC group or IgG group or Bio-probe NC group; the data were all measurement data and expressed as mean ± standard deviation; independent sample t test was used for statistical analysis between two groups, and one-way ANOVA for the comparison among multiple groups, followed by Tukey’s post hoc test; the experiment was repeated three times.