Figure 5.

SBF2-AS1 absorbs miR-30a and inhibits its expression by acting as ceRNA, thus promoting FOXA1 expression. (a) Prediction of the binding site between miR-30a and FOXA1 by Targetscan website; (b) Verification of the binding site between miR-30a and FOXA1 by dual luciferase reporter gene assay; (c) Detection of expression of SBF2-AS1, miR-30a and FOXA1 in each group after cell treatment by RT-qPCR; *, P < 0.05 vs the mimic NC group, oe-SBF2-AS1 + mimic NC group, oe-SBF2-AS1+ inhibitor NC group or miR-30a inhibitor + sh-NC group; the data were all measurement data and expressed as mean ± standard deviation; independent sample t test was used for statistical analysis between two groups, and one-way ANOVA for the comparison among multiple groups, followed by Tukey’s post hoc test; the experiment was repeated three times.