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. Author manuscript; available in PMC: 2020 Sep 10.
Published in final edited form as: Vaccine. 2019 Aug 12;37(38):5708–5716. doi: 10.1016/j.vaccine.2019.07.103

Figure 1. Single dose of rF1-V + Alhydrogel vaccine better protects female mice against pneumonic plague.

Figure 1.

Male and female mice were vaccinated with rF1-V + Alhydrogel and challenged 35 days later intranasally with 10 LD50 of virulent Y. pestis CO92 LuxPcysZK. Survival was monitored for 14 days. (A) Survival of C57BL/6 mice (compiled data from 4 independent experiments; n=18 and 14 for male and female mice, respectively). (B) Survival of BALB/c mice (data from one experiment; n=5). (C) Anti- rF1-V IgG titers from subset of mice in A and B 7 d prior to infection. (D) Ratio of IgG2c to IgG1 antibody subtypes from C57BL/6 mice. Absolute numbers (E) CD4+ T cells and (F) CD8+ T cells secreting IFN-γ from subset of C57BL/6 mice 35 d post vaccination. Survival data were analyzed by Kaplan-Meier and log-rank test and antibody and T cell phenotypes were analyzed by one-way ANOVA with Tukey post hoc analysis or student’s T-test.