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. Author manuscript; available in PMC: 2020 Sep 10.
Published in final edited form as: Vaccine. 2019 Aug 12;37(38):5708–5716. doi: 10.1016/j.vaccine.2019.07.103

Figure 4: Boost with vaccine containing Alhydrogel or SA-4-1BBL improves survival of male mice against pneumonic plague.

Figure 4:

Male C57BL/6 mice were primed with rF1-V + Alhydrogel alone (AL) or rF1-V + Alhydrogel + SA-4-1BBL (AL+BBL). 21 d later, mice were boosted with rF1-V + Alhydrogel (AL; n=12), rF1-V + SA-4-1BBL (BBL; n=8), or rF1-V + Alhydrogel + SA-4-1-BBL (AL+BBL; n=8). Absolute numbers of (A and B) CD4+ T cells and (C and D) CD8+ T cells secreting IFN-γ were determined 60 d post prime vaccination from a subset of animals. A and C are representative flow cytometer data from one animal in each group. (E) Mice were challenged intranasally 60 d post prime vaccination with 10 LD50 of virulent Y. pestis CO92 LuxPcysZK and survival was monitored for 14 d (compiled data from 3 independent experiments). (F) Anti- rF1-V IgG titers 7 d prior to infection. (G) Ratio of IgG2c to IgG1 antibody subtypes from subset of mice in D. Survival data were analyzed by Kaplan-Meier and log-rank test and cell numbers and antibody titers by one-way ANOVA with Dunnett’s post hoc analysis compared to the AL/AL prime boost group: *** = p<0.001; * = p<0.05.