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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: J Pept Sci. 2018 Nov;24(11):e3130. doi: 10.1002/psc.3130

Figure 3:

Figure 3:

Extent of deprotection of Cys(Acm) by TIS under standard deprotection conditions as measured by HPLC. (A) HPLC chromatogram of the Acm-test peptide after cleavage from the solid support using a cleavage cocktail containing TFA/TIS/H2O (96/2/2) for 2 h at room temperature. (B) HPLC chromatogram of the Acm- test peptide after further incubation of the isolated peptide in neat TFA for 2 h at room temperature. (C) HPLC chromatogram of the Acm-test peptide after further incubation of the isolated peptide in TFA/TIS (98/2) for 2 h at room temperature. Little or no deprotection is observed as evidence by the absence of change in the HPLC chromatogram.