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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: J Pept Sci. 2018 Nov;24(11):e3130. doi: 10.1002/psc.3130

Figure 5:

Figure 5:

Extent of deprotection of Cys(Mob) and Cys(But) by TIS at 37 ºC as measured by HPLC. (A) HPLC chromatogram of the Cys(Mob) test peptide after cleavage from the solid support using a cleavage cocktail containing TFA/TIS/H2O (96/2/2) for 2 h at room temperature. (B) HPLC chromatogram of the Cys(Mob) test peptide after incubation in neat TFA for 12 h at 37 ºC. (C) HPLC chromatogram of the Cys(Mob) test peptide after incubation in TFA/TIS (98/2) for 12 h at 37 ºC. (D) HPLC chromatogram of the Cys(But) test peptide after cleavage from the solid support using a cleavage cocktail containing TFA/TIS/H2O (96/2/2) for 2 h at room temperature. (E) HPLC chromatogram of the Cys(But) test peptide incubation in neat TFA for 12 h at 37 ºC. (F) HPLC chromatogram of the Cys(But) test peptide after incubation in TFA/TIS (98/2) for 12 h at 37 ºC.