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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: J Thromb Haemost. 2019 Jul 28;17(10):1733–1745. doi: 10.1111/jth.14563

Figure 2. Trauma-derived platelet extracellular vesicles decrease bleeding time and enhance thrombosis.

Figure 2.

To assess in vivo effects of PEVs released following traumatic injury, PEVs were isolated from mice subjected to polytrauma and transfused into mice undergoing tail bleed assay. Trauma-derived PEVs enhance hemostasis and decreased bleeding time (A, n=10–12/group). To reflect bleeding following trauma, we performed a liver laceration model that results in uncontrolled hemorrhage. Transfusion of 4×10^8 PEV resulted in a significant decrease in blood loss (B, n=6/group). Finally, to determine if these PEVs would also have an effect on thrombus formation, a known later complication following trauma, mice subjected to tail bleeding recovered for 24 hours and were then subjected to IVC ligation. Strikingly, mice transfused with PEVs prior to their tail vein bleed had significantly higher thrombus burden compared to control at 24 hours following IVC ligation (C, n=6–8/group). Experimental diagram of liver laceration model that results in uncontrolled intraperitoneal hemorrhage (D). *p<0.01 **p<0.05