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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Exp Physiol. 2019 Aug 8;104(10):1455–1471. doi: 10.1113/EP087499

Figure 4. Leukocyte infiltration of aorta with age, caloric restriction and voluntary running.

Figure 4.

Aortas and mesenteric vascular arcades from young normal chow (YNC), old- (O) normal chow (NC), voluntary running (VR) and calorie restricted (CR) mice were digested to a single cell suspension and incubated with antibodies against CD45 and CD3 to assess total T cells in aorta (A) and mesentery (B); CD45 and CD19 to assess total B cells in aorta (C) and mesentery (D) and; CD45, CD11b and F4/80 to assess total macrophages in aorta (E) and mesentery (F). Representative flow cytometry plots are shown on the left of each panel, summary data is shown on the right. n = 5–11/group. Differences were assessed with one-way ANOVA with LSD post hoc tests. * different from YNC, † different from ONC, p ≤ 0.05. Data are means ± SEM. Adapted with permission from Trott et al., (2018).