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Engineering of GFP-based GEZIs, ZIBG1 and ZIBG2. (a) Domain arrangements of ZIBG1 and ZIBG2. Important linker sequences are shown in individual boxes. (b) Fluorescence excitation (open circle) and emission (filled circle) spectra of ZIBG1 and ZIBG2, in the presence (blank lines, treated with 100 μM ZnCl2) and absence (gray lines, treated with 100 μM EDTA) of Zn2+.
