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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Anal Chem. 2019 Sep 10;91(19):12212–12219. doi: 10.1021/acs.analchem.9b01802

Figure 3.

Figure 3.

Fluorescence imaging of pDisplay-ZIBG2 in HEK 293T cells. (a) Fluorescence images of HEK 293T cells expressing pDisplay-ZIBG2 before and after sequential addition of ZnCl2 (100 μM) and EDTA (100 μM). Cells were co-transfected with a far-red FP, mMaroon1, which serves as an internal control. Scale bar: 20 μm. (b) Quantitative results presented as mean and s.d. of 20 cells from three independent replicates. The arrows show the time points for addition of ZnCl2 and EDTA.