Follicular fluid was aspirated on day 63 post partum from control cows (grey bar, n=19) and cows that had metritis during the first 21 days post partum (black bars, n=15). The diameter of each dominant follicle was measured (A). Follicular fluid was evaluated for estradiol (B), IL-8 (C), LPS (D), glucose (E), and cholesterol concentrations (F). Data are presented as LSM + SEM and analyzed using the generalized linear mixed model. Associations between the follicular fluid concentrations of IL-8 and LPS (G), glucose (H), or cholesterol (I) are described.