Fig. 4.

Fuzzy c-means clustering on differentially expressed features for each technique. Data was partitioned in either 32 (RNA) or 16 clusters (PROT, PHOS). A, PCA of clusters for each technique. Clusters with exceptional high increase (red), decrease (blue) or peak expression at intermediate differentiation stage (D15) (green) are highlighted. B, Expression profiles of selected RNA and PROT clusters. C, Selected clusters were filtered for transcription factors. D, Quantified and differentially expressed (DEGs) features per technique were filtered for transcription factors (TF), kinases (KIN) and phosphatases (PPT).