Table II. List of up-regulated TIF proteins secreted via classical pathway and whose mRNA levels are elevated in OSCC tissues.
| Protein name (gene name) | Log2 ratio of TIF/NIFa |
Up-regulated mRNA data set numberb | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| P01 | P02 | P04 | P05 | P10 | P12 | P13 | P14 | P15 | P17 | ||
| Chondroitin sulfate proteoglycan 4 (CSPG4) | 6.19 | 6.58 | 2.47 | 6.00 | 4.72 | 5.92 | 6.89 | 5.21 | 5.80 | 6.29 | 5/5 |
| Laminin subunit alpha-3 (LAMA3) | 5.28 | 6.17 | 4.37 | 3.73 | 5.21 | 3.51 | 4.79 | 6.11 | 7.00 | 5.42 | 5/5 |
| Laminin subunit beta-1 (LAMB1) | 5.68 | 4.36 | 4.72 | 4.11 | 3.63 | 4.59 | 1.84c | 5.25 | 4.48 | 3.57 | 5/5 |
| Laminin subunit beta-3 (LAMB3) | 5.13 | 5.93 | 4.72 | 3.85 | 5.08 | 4.38 | 4.43 | 6.32 | 6.14 | 4.88 | 5/5 |
| Laminin subunit gamma-2 (LAMC2) | 4.59 | 4.94 | 3.76 | 1.41c | 4.68 | 2.71 | 3.63 | 5.69 | 5.61 | 3.47 | 5/5 |
| Nidogen-1 (NID1) | 4.80 | 4.63 | 3.91 | 0.93c | 1.64c | 2.81 | 2.13c | 4.18 | 3.70 | 2.24 | 4/5 |
| Periostin (POSTN) | 4.72 | 2.83 | 7.09 | 4.06 | 4.92 | 2.76 | 5.68 | 4.42 | 0.80c | 2.29 | 5/5 |
| Serpin H1 (SERPINH1) | 0.94c | 3.85 | 5.63 | 5.34 | 4.77 | 3.95 | 4.11 | 2.96 | 4.65 | 2.88 | 3/5 |
| Tenascin (TNC) | 6.71 | 7.65 | 6.48 | 4.36 | 8.03 | 6.79 | 7.10 | 7.94 | 4.63 | 7.09 | 5/5 |
aThe values represent the log2 TIF/NIF ratio of normalized spectra counts in the proteome profiling of interstitial fluids.
bThe data were taken from the Oncomine 4.5 (https://www.oncomine.org/resource/login.html), including Estilo Head-Neck, Talbot Lung, and Ye Head-Neck datasets comparing between tongue squamous cell carcinoma and normal tissues, Ginos Head-Neck dataset comparing head and neck squamous cell carcinoma and normal tissues, and Peng Head-Neck dataset comparing between oral squamous cell carcinoma and normal tissues. Genes with tumor-versus-normal ratio > 1.5 and p < 0.05 were defined as overexpression in cancer tissues.
cThe protein is not upregulated in the TIF samples (p values ≥ 0.05).