Fig. 1.

Identification of potential VE-PTP substrates by anti-phosphotyrosine affinity purification after VE-PTP inhibition. A, Schematic representation of label free quantification of immunoprecipitated pY-containing and associated proteins on VE-PTP inhibitor treatment. Phosphotyrosine-containing proteins in untreated or AKB-9778-treated cells were precipitated by 4G10-antibody, separated by SDS-PAGE, in gel - digested and analyzed by label free quantitative LC-MS/MS. B, Functional interaction network of significantly enriched proteins from the immunoprecipitation experiment. Only proteins that are part of the network are displayed (40 out of 54). The size of the protein nodes reflects quantitative differences in enrichment based on label free quantification of proteins. Red label: Proteins associated with GO process signal transduction; green label: proteins associated with GO compartment cell junction.