Table 2. Minimum inhibitory concentration of donor and transconjugant, and efficiency of conjugation.
| Antimicrobialsc | Escherichia coli JM109 | 47a | 55a | 78a | 107a | 116a | 130a | 131a | 144a | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Da | T | D | T | D | T | D | T | D | T | D | T | D | T | D | T | ||
| ABPC | 4 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 |
| CEZ | 2 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 |
| CTX | <0.125 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 | >256 |
| SM | 2 | 2 | 2 | 16 | 2 | 2 | 8 | >256 | 2 | 32 | 2 | >256 | 2 | 8 | 2 | >256 | 2 |
| OTC | 2 | 256 | >256 | 4 | 2 | 2 | 2 | 4 | 2 | 2 | 4 | 4 | 2 | 4 | 2 | 4 | 2 |
| TC | 2 | 128 | 128 | 4 | 4 | 4 | 2 | 2 | 2 | 2 | 4 | 4 | 4 | 2 | 2 | 4 | 4 |
| TM | 1 | 1 | 1 | 0.5 | 1 | 1 | 0.5 | 4 | 1 | >256 | 1 | >256 | 1 | 2 | 1 | >256 | 1 |
| Efficiency of conjugation (sd)b | - | 2.2 × 10−4 | 3.0 × 10−4 | 1.6 × 10−3 | 1.1 × 10−3 | 6.7 × 10−5 | 1.9 × 10−3 | 1.5 × 10−4 | 1.4 × 10−2 | ||||||||
| (1.1 × 10−4) | (2.1 × 10−4) | (5.6 × 10−4) | (6.7 × 10−5) | (2.8 × 10−5) | (2.3 × 10−3) | (6.6 × 10−5) | (2.7 × 10−3) | ||||||||||
aD: donor, T: transconjugant. bThe efficiency of conjugation is the number of transconjugants per donor cell, from triplicate analysis in three independent experiments; sd: standard deviation. cABPC, ampicillin; CEZ, cefazolin; CTX, cefotaxime; SM, streptomycin; OTC, oxytetracycline; TC, tetracycline; TM, trimethoprim.