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. 2019 Oct 1;9:14073. doi: 10.1038/s41598-019-50671-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In situ and in vitro analysis of α_1D-adrenergic receptor C-terminal PDZ ligand:SCRIB truncation mutant interactions. (A–C). Biolayer interferometry (BLI) analyses of α_1D-CT binding to SCRIB ΔPDZ4 (A), SCRIB ΔPDZ34 (B) and SCRIB PDZ34 (C). BLI data are presented as mean ± SEM, n = 3. (D) Co-immunoprecipitation of myc-α_1D-AR with transfection vehicle (− vector), empty pGlue vector (+vector), TAP-SCRIB containing all 4 PDZ domains (WT), or sequentially truncated at the C-terminus (CT), PDZ domain 4 (ΔPDZ4), PDZ domain 3 (ΔPDZ34), PDZ domain 2 (PDZ1) or PDZ domain 1 (ΔPDZ) from HEK293 cell lysates. Shown are western blots of TAP-SCRIB constructs (top panel), myc-α_1D-AR multimers (middle panel) and monomers (bottom panel). For full blots reference Supplemental Fig. 2.