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. 2019 Oct 1;9:14109. doi: 10.1038/s41598-019-50629-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Functional complementation of GbHMGR2 and GbHMGR3 for the hmgr-deficient yeast YSC5023. (A) Functional complementation of GbHMGR2; (B) Functional complementation of GbHMGR3; (a) Diploid YSC1021 strain was grown on YPG + G418 medium; (b) Diploid YSC1021 strain was grown on YPD + G418 medium; (c) Haploid YSC5023 strain harboring pYES2-GbHMGR1 or pYES2-GbHMGR2 was grown on YPG + G418 medium; (d) Haploid YSC5023 strain harboring pYES2-GbHMGR1 or pYES2-GbHMGR2 failed to grow on YPD + G418 medium. The strains were grown at 28 °C for 2 days.