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. 2019 Oct 1;9:14050. doi: 10.1038/s41598-019-50320-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry analysis of the peritoneal exudate cells from Ehrlichia-infected mice. (A) Flow cytometry gate strategy for the analysis of the peritoneal exudate cells were carried out as in Fig. 1. CD3-negative cells were plotted as CD11b × F4/80 for the analysis of peritoneal macrophages defined as F4/80⁺CD11b⁺ cells. (B) F4/80⁺CD11b⁺ cells were also addressed for the intracellular expression of iNOS as represented by flow histograms. Values represent percentages in the assigned regions. Quantification of the analyzed cell sub-populations in the different studied groups, naive, E. muris (EM) and Ixodes Ovatus Ehrlichia (IOE) are shown in each case. Values are expressed as mean and standard deviation of percentage. Asterisks represent relevant statistical difference between groups. Data is representative of three experimental sets performed individually with n = at least three mice per group in each experimental run.