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. 2019 Oct 1;9:14114. doi: 10.1038/s41598-019-50626-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Placental tissue from pregnancies complicated by T1DM (n = 6) or T2DM (n = 6) and uncomplicated term pregnancies (maternal BMI < 30 (n = 6) and >30 (n = 6)) were stained using antibodies that recognise (A) endothelial lipase, (B) lipoprotein lipase, (C) fatty acid translocase, (D) fatty acid transporter protein (FATP) 2 or (E) FATP4 then analysed by scoring the intensity of staining in the trophoblast and endothelium as low (0), medium (1) or high (2). Data points represent the mean score from ten images, with the median value plotted as a bar. Circle - T1DM; square - T2DM; triangle - control BMI < 30; inverted triangle - control BMI > 30. Data were compared using the Kruskal-Wallis test; no differences in staining for the lipases or fatty acid transporters were observed, in either compartment, between the subject groups (p > 0.05).