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. 2019 Oct 1;9:14063. doi: 10.1038/s41598-019-50506-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Basso, Beattie, and Bresnahan (BBB) score and motor function recovery after spinal cord injury (SCI) after application of phosphate-buffered saline (PBS) or 4, 4′-diisothiocyanatostilbene-2, 2′-disulfonic acid (DIDS). (a) Graph indicating the BBB score of animals that received PBS and DIDS after injury along 6 weeks. (b) Graph showing BBB subscore of animals that received PBS and DIDS after injury along 6 weeks. Representative images of spinal cord sagittal sections from (c) controls and (d) DIDS-treated animals stained with hematoxylin and eosin (HE) after 6 weeks. Dotted line indicates the affected area. Bars represent standard error of the mean. Scale bar: 250 μm. *P < 0.05 compared with the respective time point in the PBS group. ^#P < 0.01 in the factor treatment (PBS vs. DIDS) in two-way ANOVA.