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. 2019 Oct 1;9:14134. doi: 10.1038/s41598-019-50785-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DAMTC mitigates TBI-induced apoptosis in the intestinal crypt epithelium and villus of mice. Effects of DAMTC on apoptosis in TBI mice was evaluated by TUNEL staining (brown) (counter-stain: methyl green). Micrographs show TUNEL staining of mouse small intestinal sections. Panels show representative images for naïve, DAMTC, TBI (7.6 Gy), and TBI + DAMTC groups at days (A) 3, (B) 7, (C) 14 and (D) 21 following TBI. Scale bar = 100 μm with an original magnification of x200. Enumeration of (E) apoptotic cells per five crypts and (F) apoptotic cells per villus (intestinal sections from ten animals were examined in each group; n = 10). All error bars indicate SEM. *P < 0.05; **P < 0.01; ***P < 0.001.