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. 2019 Oct 1;9:14134. doi: 10.1038/s41598-019-50785-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DAMTC induces anti-oxidant defence to reduce radiation-induced oxidative stress in the intestine of TBI mice. Effects of DAMTC on oxidative stress in TBI mice by immunoblotting analysis of anti-oxidant proteins (catalase and SOD-2) in the intestinal jejunum. Dynamics of molecular changes in naïve, DAMTC, TBI (7.6 Gy), and TBI + DAMTC mice at days (A) 3, (B) 7 and (C) 21 following TBI and treatment. Blots represented are from the same gel and β-actin served as the loading control. Membrane stripping to re-probe for a different protein was done wherever required. Quantifical analysis of immunoblots represent average relative fold change in protein levels (normalized to β-actin) with SEM between TBI (7.6 Gy) and TBI + DAMTC groups (from five independent observations). (D) Evaluation of lipid peroxidation by TBARS assay and MDA production in naïve, DAMTC, TBI (7.6 Gy), and TBI + DAMTC mice (n = 10 for each group). All error bars indicate SEM. *P < 0.05; **P < 0.01; ***P < 0.001.