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. 2019 Oct 1;9:14072. doi: 10.1038/s41598-019-50489-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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XAF1 but not MGMT promoter was demethylated in adaptive TMZ resistance GBM cells. (a) MGMT and XAF1 methylation pattern and correlation with gene expression level. (b) Methylation level of MGMT is not correlated to the expression of XAF1. (c) Schematic representation of hMGMT promoter shows the location of 25 CpG-dinucleotides sites from −336 to −646 relative to the TSS. TSS, transcription start site. (d) Bisulfite sequencing was performed of hMGMT promoter in U251, T98G control and resistant cells. (e) Schematic representation of hXAF1 promoter shows the location of 18 CpG-dinucleotides sites from + 84 to −124 and −1572 to −1781 relative to the TSS. (f) Bisulfite sequencing result of hXAF1 promoter in U251, T98G control and resistant cells. Each circle represents the average methylation of 5 clones. A hollow circle means no methylation, whereas a filled circle means 100% methylated.