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. 2019 Oct 1;9:14082. doi: 10.1038/s41598-019-50683-2

Figure 4.

Figure 4

Antigenicity, immunogenicity and efficacy of nano-iCnB evaluated in Swiss mice. (A) Inactivated alpha toxin (>250 kDa) on 11% SDS-polyacrylamide gel electrophoresis. MW – Molecular weight. Line 1, 1′; 2, 2′; 3, 3′; 4, 4′, are representing dilution of inactivated alpha toxin: pure, 1:2; 1:4 and 1:8 in duplicate; respectively. (B) Immunoblotting of immunized group (nano-iCnB). (C) Immunoblotting of individual mice serum immunized with nano-iCnB and nano-milliQW.