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. 2019 Oct 1;9:14082. doi: 10.1038/s41598-019-50683-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Survival curves of Swiss mice of experimental groups using the Kaplan-Meier method. The LT50 of the non-immunized-challenged group of 20.0 (15.1–24.9), while the LT50s of nano-milliQW and nano-iCnB were 30.0 (19.9–40.1) and 56.0 (40.8–71.2), respectively. The log rank statistic for the survival curves showed a significant difference between survival curves (p = 0.002). The survival curves were compared to the curves of the three groups (nano-milliQW, non-immunized-challenged and nano-iCnB) simultaneously, using TL50 as the comparison unit. Only animals in the nano iCnB group showed significant differences.