Table 1.
Characteristics of genes encoding LEA proteins in C. sinensis.
| Name | Gene ID | Group | No. of domain | Protein Length (aa) | MW (kDa) | pI | GRAVY | Predicted subcellular localization | Arabidopsis ortholog |
|---|---|---|---|---|---|---|---|---|---|
| CsLEA1 | CSA000844.1/TEA008269.1 | LEA_2 | 1 | 231 | 26.03 | 9.39 | 0.015 | Chloroplast | |
| CsLEA2 | CSA000980.1/TEA029076.1 | LEA_2 | 1 | 183 | 19.68 | 5.21 | 0.361 | Chloroplast | |
| CsLEA3 | CSA001281.1 | LEA_2 | 1 | 229 | 25.23 | 9.30 | 0.176 | Chloroplast | |
| CsLEA4 | CSA002179.1/TEA012563.1 | LEA_2 | 1 | 215 | 23.95 | 6.72 | −0.032 | Cytoplasmic | |
| CsLEA5 | CSA002944.1/TEA018323.1* | LEA_2 | 1 | 206 | 22.68 | 9.71 | 0.197 | Cytoplasmic | |
| CsLEA6 | CSA003226.1/TEA005685.1* | LEA_2 | 1 | 197 | 21.50 | 9.37 | 0.287 | Chloroplast | |
| CsLEA7 | CSA003726.1* | LEA_3 | 1 | 96 | 10.47 | 9.99 | −0.431 | Cytoplasmic | AT4G02380 |
| CsLEA8 | CSA004403.1/TEA010666.1* | Dehydrin | 2 | 254 | 29.01 | 5.29 | −1.470 | Nuclear | AT1G20440 |
| CsLEA9 | CSA006264.1/TEA013720.1 | LEA_3 | 1 | 106 | 11.96 | 7.09 | −0.782 | Mitochondrial | |
| CsLEA10 | CSA006938.1/TEA006416.1* | LEA_3 | 1 | 96 | 10.30 | 10.08 | −0.467 | Mitochondrial | AT4G02380 |
| CsLEA11 | CSA007094.1 | LEA_4 | 1 | 278 | 30.58 | 5.11 | −0.887 | Nuclear | AT2G42560 |
| CsLEA12 | CSA009009.1* | LEA_1 | 1 | 106 | 11.39 | 7.11 | −1.045 | Mitochondrial | AT2G35300 |
| CsLEA13 | CSA009868.1/TEA030980.1* | LEA_2 | 1 | 210 | 23.57 | 9.92 | −0.232 | Cytoplasmic | |
| CsLEA14 | CSA010151.1/TEA003016.1* | LEA_4 | 2 | 187 | 20.69 | 7.64 | −0.987 | Nuclear | AT3G53040 |
| CsLEA15 | CSA010673.1/TEA032971.1* | LEA_2 | 1 | 225 | 24.77 | 9.51 | 0.014 | Cytoplasmic | |
| CsLEA16 | CSA010820.1/TEA033104.1 | LEA_2 | 1 | 248 | 27.19 | 9.79 | −0.158 | Chloroplast | |
| CsLEA17 | CSA012431.1* | LEA_3 | 1 | 86 | 9.83 | 9.69 | −0.385 | Chloroplast | AT1G02820 |
| CsLEA18 | CSA013054.1/TEA014162.1 | LEA_2 | 1 | 250 | 27.84 | 10.01 | −0.156 | Chloroplast | |
| CsLEA19 | CSA013456.1/TEA033525.1 | LEA_2 | 1 | 210 | 23.50 | 9.58 | −0.156 | Nuclear | |
| CsLEA20 | CSA013879.1/TEA012020.1 | LEA_2 | 1 | 196 | 22.11 | 9.85 | −0.160 | Mitochondrial | |
| CsLEA21 | CSA016568.1/TEA015482.1 | LEA_2 | 1 | 271 | 29.75 | 9.75 | −0.062 | Cytoplasmic | |
| CsLEA22 | CSA019232.1* | SMP | 1 | 72 | 7.55 | 5.62 | −0.101 | Cytoplasmic | AT3G22490 |
| CsLEA23 | CSA019926.1/TEA024719.1 | LEA_2 | 1 | 451 | 49.47 | 8.81 | −0.159 | Chloroplast | |
| CsLEA24 | CSA021165.1/TEA030404.1 | LEA_2 | 1 | 228 | 25.71 | 9.23 | −0.161 | Chloroplast | |
| CsLEA25 | CSA021249.1/TEA007147.1* | LEA_2 | 1 | 211 | 23.19 | 9.66 | 0.067 | Chloroplast | |
| CsLEA26 | CSA026452.1/TEA028236.1* | LEA_1 | 1 | 142 | 14.90 | 6.13 | −0.544 | Nuclear | AT5G06760 |
| CsLEA27 | CSA027135.1 | LEA_2 | 1 | 211 | 23.51 | 9.41 | −0.029 | Plasma membrane | |
| CsLEA28 | CSA027557.1/TEA031400.1* | SMP | 2 | 213 | 22.27 | 4.72 | −0.185 | Chloroplast | AT3G22490 |
| CsLEA29 | CSA028043.1 | LEA_2 | 1 | 249 | 28.38 | 9.36 | −0.128 | Cytoplasmic | |
| CsLEA30 | CSA030792.1/TEA011337.1* | LEA_2 | 2 | 318 | 35.36 | 4.86 | −0.371 | Cytoplasmic | AT2G44060 |
| CsLEA31 | CSA030897.1/TEA008241.1 | LEA_2 | 1 | 253 | 28.05 | 9.63 | 0.051 | Cytoplasmic | |
| CsLEA32 | CSA031822.1/TEA014379.1* | LEA_2 | 1 | 153 | 16.64 | 4.93 | −0.008 | Cytoplasmic | AT1G01470 |
| CsLEA33 | CSA032382.1/TEA014165.1 | LEA_2 | 1 | 210 | 23.91 | 9.43 | 0.127 | Cytoplasmic | |
| CsLEA34 | CSA033045.1/TEA015292.1* | LEA_2 | 1 | 210 | 23.36 | 9.95 | −0.111 | Cytoplasmic | |
| CsLEA35 | CSA034141.1/TEA032830.1* | LEA_2 | 1 | 221 | 24.55 | 10.16 | 0.010 | Cytoplasmic | |
| CsLEA36 | CSA035676.1* | LEA_5 | 1 | 78 | 8.59 | 5.45 | −1.517 | Nuclear | AT3G51810 |
| CsLEA37 | CSA036017.1/TEA021072.1* | LEA_2 | 1 | 194 | 21.29 | 10.02 | −0.042 | Cytoplasmic | |
| CsLEA38 | NF | LEA_2 | 1 | 213 | 23.99 | 9.25 | 0.186 | Cytoplasmic | |
| CsLEA39 | NF | SMP | 1 | 155 | 16.89 | 9.68 | −0.741 | Chloroplast | AT3G22490 |
| CsLEA40 | TEA005489.1 | LEA_2 | 1 | 221 | 24.45 | 9.71 | 0.138 | Cytoplasmic | |
| CsLEA41 | NF | Dehydrin | 1 | 208 | 21.70 | 6.95 | −0.807 | Nuclear | AT2G21490 |
| CsLEA42 | TEA007071.1 | LEA_2 | 1 | 260 | 28.48 | 10.09 | −0.149 | Chloroplast | |
| CsLEA43 | NF | LEA_2 | 1 | 208 | 23.25 | 9.32 | 0.202 | Cytoplasmic | |
| CsLEA44 | TEA018923.1 | LEA_2 | 1 | 215 | 24.00 | 9.62 | 0.175 | Cytoplasmic | |
| CsLEA45 | TEA015290.1* | LEA_2 | 1 | 212 | 23.82 | 9.74 | −0.263 | Cytoplasmic | |
| CsLEA46 | NF | Dehydrin | 1 | 169 | 16.91 | 8.81 | −1.050 | Nuclear | AT5G66400 |
| CsLEA47 | TEA024718.1 | LEA_2 | 1 | 262 | 28.45 | 9.41 | −0.154 | Chloroplast | |
| CsLEA48 | NF | Dehydrin | 1 | 210 | 21.98 | 6.90 | −1.328 | Nuclear | AT5G66400 |
Note: NF indicates these CsLEA genes were not found in two tea plant genomes, and asterisk (*)indicates these CsLEA genes were ever reported in the previously study38.