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. 2019 Sep 25;6:89. doi: 10.3389/fmolb.2019.00089

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Copyright © 2019 Bhayani, Hill, Sharma, Iglesias, Olsen and Ballicora.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FBP saturation curves for the WT E. coli ADP-Glc PPase in the presence of varying concentrations of sulfate. Activity was measured in the presence of 1.0 mM Glc1P, 1.0 mM ATP, 7 mM MgCl₂, and 50 mM HEPES pH 7.5. The effector and sodium sulfate concentration were varied as indicated in the figure. The inset graph represents the linear trend between the calculated A_0.5 of FBP from each curve and the concentration of sulfate.