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Effect of FBP on the apparent affinity for ATP in the WT and mutants of the E. coli ADP-Glc PPase. The effect of the FBP on the ATP apparent affinity was assessed by the ratio of two different S0.5 for ATP. One was measured in absence (control) and the other in presence of 1 mM FBP. Assays were performed in the presence of 1 mM Glc1P, 10 mM MgCl2, and 50 mM HEPES pH 8.0 as indicated in section Materials and Methods.
