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. 2019 Sep 25;6:89. doi: 10.3389/fmolb.2019.00089

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Copyright © 2019 Bhayani, Hill, Sharma, Iglesias, Olsen and Ballicora.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of PLP on WT, R423A, and R52A E. coli ADP-Glc PPase activity. Activity was measured as described in Materials and Methods in the presence of 1.0 mM Glc1P, 1.5 mM ATP, 10 mM MgCl₂, and 50 mM HEPES pH 8.0. The effector concentration was varied as indicated in the figure.