Table 1.

FBP activation parameters of E. coli ADP-Glc PPase wild-type and mutants.

Enzymea	FBP
	A0.5	nH	$v_0^b$	Vmax	Vmax/v0
	(mM)		(U/mg)	(U/mg)	(-fold)
WT	0.027 ± 0.009	1.34 ± 0.17	3.3 ± 1.7	76.0 ± 0.2	23.2
R40A	N/Ac	N/Ac	1.25 ± 0.02	1.41 ± 0.01	1.1
R52A	N/Ac	N/Ac	1.76 ± 0.10	2.14 ± 0.08	1.2
R52K	N/Ac	N/Ac	1.70 ± 0.09	2.16 ± 0.06	1.3
R130A	0.067 ± 0.010	1.33 ± 0.24	63.6 ± 2.1	87.7 ± 0.6	1.4
R353A	0.040 ± 0.010	0.97 ± 0.26	5.6 ± 1.8	54.6 ± 2.9	9.8
R386A	1.00 ± 0.13	1.08 ± 0.09	2.84 ± 0.18	18.5 ± 1.0	6.5
R419A	0.036 ± 0.011	1.28 ± 0.4	11.0 ± 4.0	66.4 ± 3.1	6.0
R423A	0.32 ± 0.05	1.12 ± 0.19	7.0 ± 2.1	56.0 ± 3.8	8.0

^aAssays were performed as described in section Materials and Methods.

^bv₀ is the activity in absence of activator.

^cActivation was not significant enough to determine the parameter with precision or justify significance.