Table 2.

PLP activation parameters of E. coli ADP-Glc PPase wild type and mutants.

Enzymea	PLP
	A0.5	nH	$v_0^b$	Vmax	Vmax/v0
	(μM)		(U/mg)	(U/mg)	(-fold)
WT	0.075 ± 0.004	4.55 ± 0.67	8.1 ± 2.3	69.7 ± 0.2	8.6
R40A	0.48 ± 0.22	0.67 ± 0.29	6.8 ± 0.5	10.2 ± 0.6	1.5
R52A	N/Ac	N/Ac	7.2 ± 3.3	8.6 ± 2.8	1.2
R52K	0.22 ± 0.05	1.27 ± 0.34	4.1 ± 0.5	9.6 ± 0.3	2.3
R130A	1.29 ± 0.23	1.83 ± 0.50	56.5 ± 1.1	80.8 ± 1.8	1.4
R353A	0.16 ± 0.01	3.3 ± 0.5	10.9 ± 1.2	87.2 ± 1.3	9.3
R386A	4.2 ± 0.6	3.0 ± 0.9	15.3 ± 1.1	68.2 ± 6.3	4.5
R419A	0.019 ± 0.005	3.2 ± 1.0	11.2 ± 1.2	51.8 ± 0.9	4.6
R423A	0.21 ± 0.01	2.61 ± 0.34	8.7 ± 2.5	102.0 ± 1.4	13.5

^aAssays were performed as described in section Materials and Methods.

^bv₀ is the activity in absence of activator.

^cActivation was not significant enough to determine the parameter with precision or justify significance.