TABLE 6.

AMR gene search results by the RGI tool of the CARD Database (using contigs file as input) and AMR in vitro assay results.

Strain	RGI criteria: AMR gene	Reference gene in CARD database	Reference Cover (%) (amino acid sequence)	Identity (%) (amino acid sequence)	Bit score	AMR (in vitro assay results)∗	Genes in the vicinity of aadA gene in Prokka/RAST
L. plantarum CECT 8962	Perfect hit: aadA	WP_001206316.1	127.46	100	523.1	S	RNAi/no gene
L. plantarum CECT 8963	Strict hit: aadA2	AAF27727.1	129.73	86.72	454.5	S	RNAi/hypothetical protein
L. plantarum CECT 8964	Strict hit: aadA15	ABD58917.1	59.70	100	147.1	Amp	Only predicted by RGI-CARD
	Strict hit: aadA21	AAN87151.1	76.81	99.5	407.5		RNAi/no gene
L. plantarum CECT 8965	Perfect hit: aadA	WP_001206316.1	127.76	100	523.1	S	No gene/hypothetical protein
L. plantarum CECT 8966	Strict hit: aadA15	ABD58917.1	57.79	100	151.8	Amp	Only predicted by RGI-CARD

^∗Yépez et al. (2017); S, sensitive to all antibiotics tested; Amp, Ampicillin resistance.