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. 2019 Oct 1;9:14054. doi: 10.1038/s41598-019-50562-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ligand binding to HfqH6. Binding curves are derived from the specific change in the thermophoretic mobility upon ligand titration to a constant 100 nM concentration of HfqH6. (A) The curves show binding affinities of HfqH6 for poly(G) (open squares), poly(A) (open triangles) or RNA (filled circles). For determination of the EC50, the Hill Model was used that is included in the NT Affinity Analysis software. (B) The curves show the binding of PAP to HfqH6 in the presence (open squares) or in the absence of RNA (filled circles). Total RNA (heterologous in size and form), poly(A) and poly(G) were used with 1 kb as a crude approximation for calculation. Thereby, if we consider a 50–100 nt RNA long, the binding constants return the range typically reported for Hfq.