Table 1. Primers for quantitative real-time PCR .
Gene | Forward and Reverse primer (5′–3′) | Amplicon size (bp) |
GAPDH | F: GTGAACCATGAGAAGTATGACAAC | 123 |
R: CATGAGTCCTTCCACGATACC | ||
Oct4A | F: CTTCTCGCCCCCTCCAGGT | 496 |
R: AAATAGAACCCCCAGGGTGAGC | ||
c- Myc | F: CTCCTACGTTGCGGTCACAC | 142 |
R: CGGGTCGCAGATGAAACTCT | ||
Klf4 | F: TGCTCCCATCTTTCTCCACG | 91 |
R: TCCTGCCAGCGGTTATTCG | ||
Sox2 | F: AAGACTAGGACTGAGAGAAAGAAGAG | 171 |
R: AAGAGAGAGGCAAACTGGAATC |