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. Author manuscript; available in PMC: 2019 Oct 2.
Published in final edited form as: Acta Biomater. 2018 Jun 5;75:129–139. doi: 10.1016/j.actbio.2018.05.052

Figure 3.

Figure 3.

Protein expression during differentiation on YIGSR-aligned fibers and laminin over 14 days. Displayed images are from the 3rd quartile of fluorescent intensity for each protein. Expression is noted as typical for pluripotent state (SSEA-1, POU5F1), neural progenitors (NES, SOX1) neural (TUBB3, MAP2, GAP43) and glial cells (GFAP, OLIG1, CNPase), scale bars = 100 μm. Images indicate similar neural differentiation on (A) aligned GYIGSR-functionalized nanofiber scaffolds and (B) laminin glasses but with faster rates on fibers (earlier expression of NES, TUBB3, MAP2 and GAP43 on aligned fibers). ICC images were enhanced (+40% brightness, +20% contrast).