Table 1.
Primers used for real time qPCR with accession numbers, forward and reverse sequences.
Gene | Primer Accession Number | Forward Sequence | Reverse Sequence |
---|---|---|---|
Gapdh | NM_008084.3 | AAT GGT GAA GGT CGG TGT G | GTG GAG TCA TAC TGG AAC ATG TAG |
Actb (β-actin) | NM_007393.5 | GCT GTA TTC CCC TCC ATC GTG | CAC GGT TGG CCT TAG GGT TCA G |
Pou5f1 (Oct-4) | NM_013633.3 | GGC ACT TCA GAA ACA TGG TCT | GAA GCC GAC AAC AAT GAG AAC |
Sox1 | NM_009233 | GGC AGT CAT ACA AAA GTT GGC | GTA CAG TAT TTA TCG TCC GCA GA |
Pax6 | NM_001310144.1 | AAG GGC GGT GAG CAG ATG T | CAT GCT GGA GCT GGT TGG |
Nes | NM_016701.3 | CAC CTC AAG ATG TCC CTT AGT C | GGA AAG CCA AGA GAA GCC T |
Tubb3 | NM_023279.2 | CCT CCG TAT AGT GCC CTT TG | GTG GAC TTG GAA CCT GGA AC |
Map2 | NM_001039934.1 | CCA CTA ATG CCA GTT TCT CTC T | GAC CCA GAG TGT GTG AGT TTA T |
Gfap | NM_010277.3 | CCA CCA GTA ACA TGC AAG AGA | GCG ATA GTC GTT AGC TTC GTG |
Th | NM_009377.1 | CCC TAC CAA GAT CAA ACC TAC C | CTG GAT ACG AGA GGC ATA GTT C |
Foxo4 | NM_018789.2 | GCTCTTGGTGGATGCTGAAC | AACTGCTTCGTGGACGGAAA |
Cdh2 (n-cadherin) | NM_007664 | GCCCGCTATTTGTTACCAGC | CACAGACGCCT GAAGCAAGG |
Gap43 | NM_008083.2 | AGG AGG AGA AAG ACG CTG TA | TCA GGC ATG TTC TTG GTC AG |
Syp | NM_009305.2 | TTT GGA GGG TGA GCG AAA TG | AGA GAA AGG GTG GAG AAG GTA G |
Olig1 | NM_016968 | TCC AGA CTT CTC TCC CAG AC | AGC AAC TAC ATC GCT CCT TG |