Skip to main content
. 2019 Jun 12;317(3):G314–G332. doi: 10.1152/ajpgi.00104.2019

Fig. 1.

Fig. 1.

Expression of nucleoside triphosphate diphosphohydrolase-1 and -2 (NTPDase1 and -2) within the gut wall. A: cross sections of distal colons from wild-type (WT; top row), Entpd1−/− (middle row), and Entpd2−/− (bottom row) mice were stained for NTPDase1 (magenta), NTPDase2 (green), pan-leukocyte marker CD45 (cyan), and glial fibrillary acidic protein (GFAP, blue), a marker of enteric glia. NTPDase1-immunoreactivity (ir) colocalized with immune cells (arrowheads) and NTPDase2-ir with enteric glia (arrows). Both the enzymes are also expressed by other cell types in the gut wall (see text). CM, circular muscle; DIC, differential interference contrast; LM, longitudinal muscle; M, mucosa; MP, myenteric plexus; SM, submucosa; SMP submucosal plexus. B: expression of NTPDase1 and NTPDase2 within the myenteric plexus. Longitudinal muscle-myenteric plexus preparations of distal colons from WT (top row), Entpd1−/− (middle row), and Entpd2−/− (bottom row) mice were stained for NTPDase1 (magenta), NTPDase2 (green), CD45 (cyan), and GFAP (blue). Scale bars = 50 µm. These are representative images from 4 to 5 mice per group.