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Ninjurin2 is localized on the plasma membrane. Immunocytochemical localization of ninjurin2 on wild-type CHO cells (A) and CHO cells transfected with pNINJ2 (B–D). Anti-ninjurin2 antibodies were applied to cells either after fixation with 4% paraformaldehyde (A, B) or before fixation (C). In D, anti-ninjurin2 antisera were blocked by incubation with the peptide immunogen and applied to cells as in B (see Materials and Methods). Scale bar, 40 μm.
