AMPA exposure causes mitochondrial depolarization in motor neurons. TMRE-loaded spinal cultures were exposed to AMPA (50 μm for 10 min). Fluorescent images were obtained before (A), during (8 min; B), and 30 min after termination of (C) the exposure. After imaging, the culture was stained for SMI-32 (D). Note the relatively selective and long-lasting decrease in TMRE fluorescence seen in the mitochondrial-rich perinuclear regions of the motor neuron, indicative of significant mitochondrial depolarization. The pseudocolorbar shows the eight-bit intensity scale used for TMRE fluorescence. Scale bar, 50 μm.