Fig. 8.

Kainate exposure triggers preferential ROS generation in motor neurons. HEt-loaded cultures were imaged for 10 min before and 25 min after addition of kainate (100 μm + 10 μm MK-801). To assess involvement of mitochondria in the ROS generation, in some cultures, we added the electron transport chain inhibitor rotenone (10 μm) for 40 min before and during the kainate exposure (broken line). After exposure, cultures were fixed and stained for SMI-32. Fluorescence changes for each neuron are expressed as the ratio of fluorescence at each time point (F_X) to its own baseline (F₀). Traces represent the means ± SEM of 7–10 motor neurons and >100 other spinal neurons derived from at least six experiments.