Fig. 9.

AMPA/kainate receptor activation causes greater ROS generation in motor neurons than in GABAergic cortical neurons. HEt-loaded spinal (A, B) and cortical (C, D) cultures were imaged for 5 min before and 30 min after a 10 min exposure to AMPA (50 μm + 10 μm MK-801; A, C) or kainate (100 μm + 10 μm MK-801; B, D).Traces represent the means ± SEM compiled from 10 to 12 motor and 200 other spinal neurons and from 60 GABAergic and 200 other cortical neurons from at least six experiments. Note the substantially greater fluorescence increase in motor neurons than in GABAergic neurons after AMPA exposure in contrast to the comparable fluorescence increases seen after kainate exposure (p < 0.01 by two-tailed ttest).