Table 1.
Detailed statistical analysis of tamoxifen treatment on WT SHANK3 protein expression
| Biochemistry | |||||
|---|---|---|---|---|---|
| 15-dTAM s.c. vsVeh s.c. (Cre+) | Two-way ANOVA | GenotypeTreatmentInteraction | F(2,36) = 20.97F(1,36) = 1.61F(2,36) = 0.49 | *p < 0.0001p = 0.2133p = 0.6194 | |
| (Fig. 4A) | Tukey HSD | Veh Shank3+/+ vsVeh Shank3+/+vs Veh Shank3+/G vsVeh Shank3+/+vsVeh Shank3+/+vsVeh Shank3+/+vsTam Shank3+/+vsTam Shank3+/+vsTam Shank3+/Gvs | Veh Shank3+/G Veh Shank3G/G Veh Shank3G/G Tam Shank3+/+ Tam Shank3+/G Tam Shank3G/G Tam Shank3+/G Tam Shank3G/G Tam Shank3G/G | p = 0.0705*p = 0.0003p = 0.2997p = 1.0000p = 0.6835*p = 0.0024p = 0.6835*p = 0.0241p = 0.0998 | |
| One-weekTAM diet vs Veh diet (Cre+) | Two-way ANOVA | GenotypeTreatmentInteraction | F(2,36) = 25.49F(1,36) = 1.78F(2,36) = 0.45 | *p < 0.0001p = 0.1906p = 0.6425 | |
| (Fig. 4B) | Tukey HSD | Veh Shank3+/+vs Veh Shank3+/+vs Veh Shank3+/G vsVeh Shank3+/+vsVeh Shank3+/+vsVeh Shank3+/+vsTam Shank3+/+vsTam Shank3+/+vsTam Shank3+/Gvs | Veh Shank3+/G Veh Shank3G/G Veh Shank3G/G Tam Shank3+/+ Tam Shank3+/G Tam Shank3G/G Tam Shank3+/G Tam Shank3G/G Tam Shank3G/G | *p = 0.0447*p = 0.0002p = 0.1242p = 1.0000p = 0.3864*p = 0.0012p = 0.3864*p = 0.0012p = 0.1548 | |
| Two-weekTAM diet vs Veh diet (Cre+) | Two-way ANOVA | GenotypeTreatmentInteraction | F(2,36) = 18.64F(1,36) = 4.92F(2,36) = 1.49 | *p < 0.0001*p = 0.0329p = 0.2389 | |
| (Fig. 4B) | Tukey HSD | Veh Shank3+/+vs Veh Shank3+/+vs Veh Shank3+/G vsVeh Shank3+/+vsVeh Shank3+/+vsVeh Shank3+/+vsTam Shank3+/+vsTam Shank3+/+vsTam Shank3+/Gvs | Veh Shank3+/G Veh Shank3G/G Veh Shank3G/G Tam Shank3+/+ Tam Shank3+/G Tam Shank3G/G Tam Shank3+/G Tam Shank3G/G Tam Shank3G/G | p = 0.0523*p = 0.0002p = 0.1395p = 1.0000p = 0.6185*p = 0.0404p = 0.6185*p = 0.0404p = 0.6526 | |
| Four-weekTAM diet vs Veh diet (Cre+) | Two-way ANOVA | GenotypeTreatmentInteraction | F(2,36) = 13.70F(1,36) = 5.73F(2,36) = 1.55 | *p < 0.0001*p = 0.0220p = 0.2262 | |
| (Fig. 4B) | Tukey HSD | Veh Shank3+/+vs Veh Shank3+/+vs Veh Shank3+/G vsVeh Shank3+/+vsVeh Shank3+/+vsVeh Shank3+/+vsTam Shank3+/+vsTam Shank3+/+vsTam Shank3+/Gvs | Veh Shank3+/G Veh Shank3G/G Veh Shank3G/G Tam Shank3+/+ Tam Shank3+/G Tam Shank3G/G Tam Shank3+/G Tam Shank3G/G Tam Shank3G/G | p = 0.1104*p = 0.0004p = 1.0000p = 1.0000p = 0.9380p = 0.1530p = 0.9380p = 0.1530p = 0.6222 | |
| Six-weekTAM diet vsVeh diet (Cre-) | Two-way ANOVA | GenotypeTreatmentInteraction | F(2,44) = 45.29F(1,44) = 0.17F(2,44) = 0.39 | *p < 0.0001p = 0.6783p = 0.7010 | |
| (Fig. 4C) | Tukey HSD | Veh Shank3+/+vs Veh Shank3+/+vs Veh Shank3+/G vsVeh Shank3+/+vsVeh Shank3+/+vsVeh Shank3+/+vsTam Shank3+/+vsTam Shank3+/+vsTam Shank3+/Gvs | Veh Shank3+/G Veh Shank3G/G Veh Shank3G/G Tam Shank3+/+ Tam Shank3+/G Tam Shank3G/G Tam Shank3+/G Tam Shank3G/G Tam Shank3G/G | *p = 0.0008*p = 0.0001p = 0.2915p = 0.9236*p = 0.0007*p = 0.0001*p = 0.0046*p = 0.0001*p = 0.0255 | |
| Six-weekTAM diet vsVeh diet (Cre+) | Two-way ANOVA | GenotypeTreatmentInteraction | F(2,112) = 7.81F(1,112) = 14.67F(2,112) = 4.87 | *p < 0.0001*p = 0.0002*p = 0.0094 | |
| (Fig. 4D) | Tukey HSD | Veh Shank3+/+vs Veh Shank3+/+vs Veh Shank3+/G vsVeh Shank3+/+vsVeh Shank3+/+vsVeh Shank3+/+vsTam Shank3+/+vsTam Shank3+/+vsTam Shank3+/Gvs | Veh Shank3+/G Veh Shank3G/G Veh Shank3G/G Tam Shank3+/+ Tam Shank3+/G Tam Shank3G/G Tam Shank3+/G Tam Shank3G/G Tam Shank3G/G | p = 0.1319*p = 0.0005*p = 0.0012p = 1.0000p = 0.7763p = 0.8507p = 0.6913p = 0.9308p = 0.2261 | |
*
Significant at P < 0.05 level.